Mass Spectral Studies Reveal the Structure of Aβ1–16–Cu2+ Complex Resembling ATCUN Motif
journal contributionposted on 2012-08-06, 00:00 authored by Yamini P. Ginotra, Shefali N. Ramteke, Rapole Srikanth, Prasad P. Kulkarni
In Alzheimer’s disease, copper binds to amyloid beta (Aβ) peptide and generates oxidative stress. The coordination of histidine (His) residues to Cu2+ is still uncertain. We studied Cu2+ binding to Aβ1–16 peptide using the diethyl pyrocarbonate (DEPC) assay and mass spectrometry. Our results show that only one His is involved in Cu2+ coordination, which is identified as His6 using mass spectral studies. Novel nickel displacement studies have further supported the proposal that the Cu2+ binding site of Aβ1–16 peptide resembles the ATCUN motif of human serum albumin.