posted on 2021-11-29, 16:15authored byNick Tomczyk, Kevin Giles, Keith Richardson, Jakub Ujma, Martin Palmer, Peter Kresten Nielsen, Kim F. Haselmann
The
identification and localization of isomeric peptide modifications
is a critical requirement of the biopharmaceutical industry. Despite
the ability of liquid chromatography–mass spectrometry to identify
many of the common post translational modifications, the identification
of isobaric or racemized peptides is confounded by modern mass spectrometry-based
techniques. Here, we present a novel approach combining liquid chromatography
with a high-resolution ion mobility mass spectrometry system to differentiate
peptide and peptide fragments based upon their mobility and mass.