Manipulating Mesenchymal Stem Cell Differentiation on Nanopattern Constructed through Cell-Mediated Mineralization

The extracellular matrix microenvironment, including chemical constituents and topological structure, plays key role in regulating the cell behavior, such as adhesion, proliferation, differentiation, apoptosis, etc. Until now, to investigate the relationship between surface texture and cell response, various ordered patterns have been prepared on the surface of different matrixes, whereas almost all these strategies depend on advanced instruments or severe synthesis conditions. Herein, cell-mediated mineralization method has been applied to construct nanopattern on the surface of β-TCP scaffold. The formation process, morphology, and composition of the final pattern were characterized, and a possible mineralization mechanism has been proposed. Moreover, the cell behavior on the nanopattern has been investigated, and the results showed that the mouse bone marrow mesenchyme stem cells (mBMSCs) display good affinity with the nanopattern, which was manifested by the good proliferation and osteogenic differentiation status of cells. The synthetic strategy may shed light to construct advanced topological structures on other matrixes for bone repair.