A Fast and Efficient Method for Detecting H₂O₂ by a Dual-Locked Model Chemosensor

A pentafluorobenzene-containing fluorescent probe GW-1 was designed and synthesized for monitoring hydrogen peroxide. The probe’s fluorescence was activated by a dual-locked model system that consists of a spiro location and a target analyte, which avoids the “alkalizing effect.” The smart GW-1 exhibited high selectivity toward hydrogen peroxide over other reactive oxygen species (ROS) by a dual-controlled molecular switch. These features are favorable for H₂O₂ sensing and pH changes in bioanalytical and biomedical applications.