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Listeria monocytogenes Inoculation Impedes the Development of Brain Pathology in Experimental Cerebral Malaria by Inhibition of Parasitemia
journal contribution
posted on 2022-04-01, 12:38 authored by Ji Yang, Xue Han, Kang-Ning Gao, Zan-Mei QiCerebral malaria (CM) is a serious
central nervous system dysfunction
caused by Plasmodium falciparum infection.
In this study, we investigated the effect of Listeria
monocytogenes (Lm) inoculation on
experimental cerebral malaria (ECM) using Plasmodium
berghei ANKA (PbA)-infected C57BL/6
mice. Live Lm inoculation inhibited the parasitemia
and alleviated ECM symptoms. The protective effect against ECM symptoms
was connected with improved brain pathology manifested as a less-damaged
blood–brain barrier, decreased parasite sequestration, and
milder local inflammation. Meanwhile, Lm inoculation
decreased expression of cell
adhesion molecules (ICAM-1 and VCAM-1) and accumulation of pathogenic
CD8+ T cells in the brain. In keeping with the suppression
of parasitemia, there was an upregulation of IFN-γ, IL-12, MCP-1,
and NO expression in the spleen by Lm inoculation
upon PbA infection. Early treatment with exogenous
IFN-γ exhibited a similar effect to Lm inoculation
on PbA infection. Taken together, Lm inoculation impedes the development of brain pathology in ECM, and
early systemic IFN-γ production may play a critical role in
these protective effects.
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sup >+ supmilder local inflammationlisteria monocytogenes (<experimental cerebral malariadecreased parasite sequestrationcell adhesion molecules)- infected c57blplasmodium falciparum infectionparasitemia cerebral malariaearly systemic ifnalleviated ecm symptomsinoculation decreased expressionecm symptomsearly treatmenttaken togetherprotective effectspb pathogenic cd8lm inoculation uponinoculation inhibitedinoculation impedesexogenous ifncritical role>) inoculation6 mice