Lissoclibadin 1, a Polysulfur Aromatic Alkaloid from the Indonesian Ascidian Lissoclinum cf. badium, Induces Caspase-Dependent Apoptosis in Human Colon Cancer Cells and Suppresses Tumor Growth in Nude Mice
journal contributionposted on 09.02.2017, 13:34 by Takeo Tatsuta, Masahiro Hosono, Henki Rotinsulu, Defny S. Wewengkang, Deiske A. Sumilat, Michio Namikoshi, Hiroyuki Yamazaki
Lissoclibadins, polysulfur aromatic alkaloids, were isolated from the Indonesian ascidian Lissoclinum cf. badium. Lissoclibadins 1 (1), 3 (2), 4 (3), 7 (4), 8 (5), and 14 (6) inhibited the growth of four human solid cancer cell lines: HCT-15 (colon adenocarcinoma), HeLa-S3 (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma), and NCI-H28 (mesothelioma). Lissoclibadin 1 (1) exerted the most potent cytotoxic effects in vitro and mainly promoted apoptosis through an intrinsic pathway with the activation of a caspase-dependent pathway in HCT-15 cells. In vivo studies demonstrated that 1 suppressed tumor growth in nude mice carrying HCT-15 cells without significant secondary adverse effects. In conclusion, the results obtained in the present study demonstrate that 1 has potential as a chemotherapeutic candidate for preclinical investigations.
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HCT -15 cellsNCI-H 28Induces Caspase-Dependent ApoptosisHuman Colon Cancer CellsPolysulfur Aromatic AlkaloidIndonesian ascidian Lissoclinum cftumor growthNude Mice Lissoclibadinsvivo studiesadenocarcinomaIndonesian Ascidian Lissoclinum cfcaspase-dependent pathwaybadiumchemotherapeutic candidatecancer cell linesLissoclibadins 1Lissoclibadin 1HCT -15Suppresses Tumor Growthcytotoxic effectsMCF