Liposome–Quantum Dot Complexes Enable Multiplexed Detection of Attomolar DNAs without Target Amplification
journal contributionposted on 13.02.2013, 00:00 by Juan Zhou, Qiang-xin Wang, Chun-yang Zhang
Sensitive detection of DNA usually relies on target amplification approaches such as polymerase chain reaction and rolling circle amplification. Here we describe a new approach for sensitive detection of low-abundance DNA using liposome–quantum dot (QD) complexes and single-particle detection techniques. This assay allows for detection of single-stranded DNA at attomolar concentrations without the involvement of target amplification. Importantly, this strategy can be employed for simultaneous detection of multiple DNA targets.