mp3001099_si_001.pdf (250.75 kB)
Lipase Is Essential for the Study of in Vitro Release Kinetics from Organogels
journal contribution
posted on 2016-02-20, 20:30 authored by Marie-Hélène Dufresne, Elaheh Marouf, Yvonne Kränzlin, Marc A. Gauthier, Jean-Christophe LerouxIn vitro drug release studies remain
indispensable
in the development of drug delivery systems, even if correlations
between in vitro and in vivo results
are often imperfect. In this work, an improved in vitro analysis method for studying in situ-forming lipid-based
implants was developed. More specifically, lipase was found to be
an essential additive for evidencing differences in drug release kinetics
from organogels of different amino acid-based organogelators,
organogelator concentrations, drug loadings, and volumes. Lipases
are thought to participate in the degradation of and release from
amino acid-based organogel implants in vivo. Our
experimental conditions allowed for the rapid and reliable screening
of in vitro parameters that may be optimized to slow
or accelerate drug release, once preliminary in vivo data are available.