sb0c00380_si_001.pdf (607.96 kB)
Leveraging Oxidative Stress to Regulate Redox Balance-Based, In Vivo Growth Selections for Oxygenase Engineering
journal contributionposted on 2020-10-06, 13:35 authored by Sarah Maxel, Edward King, Yulai Zhang, Ray Luo, Han Li
Directed evolution methods based on high-throughput growth selection enable efficient discovery of enzymes with improved function in vivo. High-throughput selection is particularly useful when engineering oxygenases, which are sensitive to structural perturbations and prone to uncoupled activity. In this work, we combine the principle that reactive oxygen species (ROS) produced by uncoupled oxygenase activity are detrimental to cell fitness with a redox balance-based growth selection method for oxygenase engineering that enables concurrent advancement in catalytic activity and coupling efficiency. As a proof-of-concept, we engineered P450-BM3 for degradation of acenaphthene (ACN), a recalcitrant environmental pollutant. Selection of site-saturation mutagenesis libraries in E. coli strain MX203 identified P450-BM3 variants GVQ-AL and GVQ-D222N, which have both improved coupling efficiency and catalytic activity compared to the starting variant. Computational modeling indicates that the discovered mutations cooperatively optimize binding pocket shape complementarity to ACN, and shift the protein’s conformational dynamics to favor the lid-closed, catalytically competent state. We further demonstrated that the selective pressure on coupling efficiency can be tuned by modulating cellular ROS defense mechanisms.
evolution methodsP 450-BMsite-saturation mutagenesis librariesHigh-throughput selectioncell fitnessGVQ-D 222NOxygenase Engineeringoxygenase engineeringefficiencyROS defense mechanismsreactive oxygen speciesLeveraging Oxidative Stressuncoupled activityACNP 450-BM variants GVQ-ALhigh-throughput growth selectionComputational modeling. coliRegulate Redox Balance-BasedVivo Growth SelectionsE . coli strain MX 203uncoupled oxygenase activityengineering oxygenasesredox balance-based growth selectio...binding pocket shape complementarity