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Laser Treated Carbon Nanotube Yarn Microelectrodes for Rapid and Sensitive Detection of Dopamine in Vivo
journal contribution
posted on 2016-02-26, 00:00 authored by Cheng Yang, Elefterios Trikantzopoulos, Michael D. Nguyen, Christopher B. Jacobs, Ying Wang, Masoud Mahjouri-Samani, Ilia N. Ivanov, B. Jill VentonCarbon
nanotube yarn microelectrodes (CNTYMEs) exhibit rapid and
selective detection of dopamine with fast-scan cyclic voltammetry
(FSCV); however, the sensitivity limits their application in vivo.
In this study, we introduce laser treatment as a simple, reliable,
and efficient approach to improve the sensitivity of CNTYMEs by threefold
while maintaining high temporal resolution. The effect of laser treatment
on the microelectrode surface was characterized by scanning electron
microscopy, Raman spectroscopy, energy dispersion spectroscopy, and
laser scanning confocal microscopy. Laser treatment increases the
surface area and oxygen containing functional groups on the surface,
which provides more adsorption sites for dopamine than at unmodified
CNTYMEs. Moreover, similar to unmodified CNTYMEs, the dopamine signal
at laser treated CNTYMEs is not dependent on scan repetition frequency,
unlike the current at carbon fiber microelectrodes (CFMEs) which decreases
with increasing scan repetition frequency. This frequency independence
is caused by the significantly larger surface roughness which would
trap dopamine-o-quinone and amplify the dopamine
signal. CNTYMEs were applied as an in vivo sensor with FSCV for the
first time, and laser treated CNTYMEs maintained high dopamine sensitivity
compared to CFMEs with an increased scan repetition frequency of 50
Hz, which is 5-fold faster than the conventional frequency. CNTYMEs
with laser treatment are advantageous because of their easy fabrication,
high reproducibility, fast electron transfer kinetics, high sensitivity,
and rapid in vivo measurement of dopamine and could be a potential
alternative to CFMEs in the future.
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carbon fiber microelectrodesenergy dispersion spectroscopyVivo Carbon nanotube yarn microelectrodeslaser scanning confocal microscopysensitivityscan repetition frequencylaser Treated Carbon Nanotube Yarn Microelectrodeselectron transfer kineticsFSCVscanning electron microscopyvivolaser treatmentlaser treatment increasessurfacedopamine signalCNTYMECFME
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