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Label-Free Luminescent Switch-On Probe for Ochratoxin A Detection Using a G‑Quadruplex-Selective Iridium(III) Complex

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journal contribution
posted on 2015-04-22, 00:00 authored by Lihua Lu, Modi Wang, Li-Juan Liu, Chung-Hang Leung, Dik-Lung Ma
A library of six luminescent Ir­(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir­(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.