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Label-Free Electrochemical Detection of Protein Based on a Ferrocene-Bearing Cationic Polythiophene and Aptamer

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journal contribution
posted on 01.07.2006, 00:00 by Fabien Le Floch, Hoang A. Ho, Mario Leclerc
Two label-free electrochemical methods for the detection of human α-thrombin using a water-soluble, ferrocene-functionalized polythiophene transducer and a single-stranded oligonucleotide aptamer probe are described. The first approach is a direct method in which the recorded current decreases upon addition of the targeted protein. The second one requires more steps and the additional utilization of PNA probes and nuclease enzyme. This indirect method leads to an increase of the electrical signal as a function of the concentration of human α-thrombin with a detection limit of 75 fmol.