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Label-Free Detection of DNA Hybridization Using Surface Enhanced Raman Spectroscopy

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journal contribution
posted on 22.09.2010, 00:00 by Aoune Barhoumi, Naomi J. Halas
The SERS spectrum of DNA is strongly dominated by the strong spectral feature of adenine at 736 cm−1; the presence of adenine can serve as an endogenous marker for the label-free SERS-based detection of DNA hybridization when the probe DNA sequence is adenine-free. The substitution of 2-aminopurine for adenine on the probe DNA sequence enables the detection of a target sequence using SERS, upon hybridization of the target with the 2-AP-substituted probe DNA sequence.