bc1005027_si_001.pdf (510.63 kB)

LNA for Optimization of Fluorescent Oligonucleotide Probes: Improved Spectral Properties and Target Binding

Download (510.63 kB)
journal contribution
posted on 20.04.2011, 00:00 by Irina V. Astakhova, Alexey V. Ustinov, Vladimir A. Korshun, Jesper Wengel
Mixmer LNA/DNA fluorescent probes containing the 1-(phenylethynyl)pyrene fluorophore attached to 2′-arabino-uridine were synthesized and studied. The conjugates displayed significantly higher hybridization affinity to target DNA, increased fluorescence quantum yields of single-stranded oligonucleotides and their duplexes, and improved ability to form an interstrand excimer compared to analogous non-LNA probes.