nn8b04639_si_001.pdf (5.46 MB)
Download fileKinetic Trans-Assembly of DNA Nanostructures
journal contribution
posted on 2018-08-16, 00:00 authored by Jihoon Shin, Junghoon Kim, Sung Ha Park, Tai Hwan HaThe central dogma of molecular biology
is the principal framework for understanding how nucleic acid information
is propagated and used by living systems to create complex biomolecules.
Here, by integrating the structural and dynamic paradigms of DNA nanotechnology,
we present a rationally designed synthetic platform that functions
in an analogous manner to create complex DNA nanostructures. Starting
from one type of DNA nanostructure, DNA strand displacement circuits
were designed to interact and pass along the information encoded in
the initial structure to mediate the self-assembly of a different
type of structure, the final output structure depending on the type
of circuit triggered. Using this concept of a DNA structure “trans-assembling”
a different DNA structure through nonlocal strand displacement circuitry,
four different schemes were implemented. Specifically, 1D ladder and
2D double-crossover (DX) lattices were designed to kinetically trigger
DNA circuits to activate polymerization of either ring structures
or another type of DX lattice under enzyme-free, isothermal conditions.
In each scheme, the desired multilayer reaction pathway was activated,
among multiple possible pathways, ultimately leading to the downstream
self-assembly of the correct output structure.