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Kinetic Analysis of Superoxide Anion Radical-Scavenging and Hydroxyl Radical-Scavenging Activities of Platinum Nanoparticles

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journal contribution
posted on 15.07.2008, 00:00 by Takeki Hamasaki, Taichi Kashiwagi, Toshifumi Imada, Noboru Nakamichi, Shinsuke Aramaki, Kazuko Toh, Shinkatsu Morisawa, Hisashi Shimakoshi, Yoshio Hisaeda, Sanetaka Shirahata
There are few reports on the physiological effects of metal nanoparticles (nps), especially with respect to their functions as scavengers for superoxide anion radical (O2·−) and hydroxyl radical (·OH). We tried to detect the scavenging activity of Pt nps using a hypoxanthine−xanthine oxidase system for O2·− and using a Fenton and a UV/H2O2 system for ·OH. Electron spin resonance analysis revealed that 2 nm particle size Pt nps have the ability to scavenge O2·− and ·OH. The calculated rate constant for the O2·−-scavenging reaction was 5.03 ± 0.03 × 107 M−1 s−1. However, the analysis of the Fenton and UV/H2O2 system in the presence of Pt nps suggested that the ·OH-scavenging reaction cannot be determined in both systems. Among particle sizes tested from 1 to 5 nm, 1 nm Pt nps showed the highest O2·−-scavenging ability. Almost no cytotoxicity was observed even after adherent cells (TIG-1, HeLa, HepG2, WI-38, and MRC-5) were exposed to Pt nps at concentrations as high as 50 mg/L. Pt nps scavenged intrinsically generated reactive oxygen species (ROS) in HeLa cells. Additionally, Pt nps significantly reduced the levels of intracellular O2·− generated by UVA irradiation and subsequently protected HeLa cells from ROS damage-induced cell death. These findings suggest that Pt nps may be a new type of antioxidant capable of circumventing the paradoxical effects of conventional antioxidants.

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