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Download fileIsolation of anteiso-C17, iso-C17, iso-C16, and iso-C15 Bacillomycin D from Bacillus amyloliquefaciens SD-32 and Their Antifungal Activities against Plant Pathogens
journal contribution
posted on 2014-02-19, 00:00 authored by Keijitsu Tanaka, Atsushi Ishihara, Hiromitsu NakajimaTwo new cyclic lipopeptides (3 and 4)
were isolated from the culture filtrate of Bacillus
amyloliquefaciens strain SD-32, together with two
known metabolites, iso-C15 and iso-C16 bacillomycin D (1 and 2). Spectroscopic and chemical analyses identified the structures
of the new compounds 3 and 4 as anteiso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-14-methylhexadecanoic acid) and iso-C17 bacillomycin D, cyclic (l-Asn-d-Tyr-d-Asn-l-Pro-l-Glu-d-Ser-l-Thr-3-amino-15-methylhexadecanoic acid), respectively.
The absolute configuration of C-3 in the β-amino fatty acid
was determined to be R on the basis of the CD spectrum
of its dinitrophenyl-p-methoxyaniline derivative.
The activities of compounds 1–4 were
evaluated against 13 plant pathogens: the activities of anteiso- and iso-C17 bacillomycin D (3 and 4) were almost the same and stronger than those
of iso-C15 and iso-C16 bacillomycin D (1 and 2); iso-C15 bacillomycin D (1) was weakest.
Compounds 2–4 inhibited the growth
of all fungi tested; however, Pythium aphanidermatum was not inhibited at all by any of the compounds. Furthermore, compounds 1–4 at concentrations of 80, 40, 30, and
30 μM, respectively, inhibited completely the Botrytis cinerea infection in cucumber leaf.