posted on 2021-03-18, 16:34authored byMeng Li, Liu Huang, Joyce Chen, Fangfang Ni, Yating Zhang, Fei Liu
Exosomes
are nanoscale (30–150 nm) biological vesicles that
are actively released from living cells and circulating into all body
fluids. Recently, exosomes in cerebrospinal fluid (CSF) have been
recognized as promising biomarkers for central nervous system (CNS)
neoplasms. In this study, we report a label-free method that can be
used to rapidly isolate exosomes from CSF for proteomic analysis.
Compared to ultracentrifugation and polyethylene glycol-based precipitation,
our method isolates exosomes from 2 mL of CSF within 10 min, which
is 18 times and 72 times shorter, respectively; the yield was increased
by 4.47 times and 2.09 times and the purity was increased by 4.54
times and 9.76 times, respectively. The proteomic analysis further
revealed that the exosomes isolated by our method identified more
exosome-related proteins, which may reflect the physiological status
of diseases for exosome-based diagnosis. Therefore, the effective
isolation of pure exosomes from CSF samples for protein analysis will
benefit the downstream analysis and clinical translation of exosomes,
thus promoting the early diagnosis of CNS neoplasms.