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Isolation and Structure Elucidation of Cystargamide, a Lipopeptide from Kitasatospora cystarginea

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journal contribution
posted on 27.06.2014, 00:00 by Krista A. Gill, Fabrice Berrué, Jennifer C. Arens, Russell G. Kerr
A new lipopeptide, cystargamide (1) was isolated from the fermentation broth of the actinomycete Kitasatospora cystarginea. The bacterial strain was selected from a set of 12 Kitasatospora spp. using a secondary metabolomics approach combining liquid chromatography/high-resolution mass spectrometry (LC–HRMS) with principal component analysis (PCA). Cystargamide (1) was purified by reversed-phase HPLC, and the structure elucidation was achieved by interpreting mass spectrometry and NMR data. Cystargamide (1) contains rare structural features including a 5-hydroxy tryptophan residue and a 2,3-epoxydecanoyl fatty acid group.