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Investigating the Ruthenium Metalation of Proteins: X‑ray Structure and Raman Microspectroscopy of the Complex between RNase A and AziRu
journal contribution
posted on 2013-10-07, 00:00 authored by Alessandro Vergara, Irene Russo Krauss, Daniela Montesarchio, Luigi Paduano, Antonello MerlinoA Raman-assisted
crystallographic study on the adduct between AziRu, a Ru(III) complex
with high antiproliferative activity, and RNase A is presented. The
protein structure is not perturbed significantly by the Ru label.
The metal coordinates to ND atoms of His105 or of His119 imidazole
rings, losing all of its original ligands but retaining octahedral,
although distorted, coordination geometry. The AziRu binding inactivates
the enzyme, suggesting that its antitumor action can be exerted by
a mechanism of competitive inhibition.