posted on 2024-03-09, 14:05authored byIbrar Ahmad, Muhammad Nadeem Khan, Khizar Hayat, Tanveer Ahmad, Dilawar Farhan Shams, Waliullah Khan, Vineet Tirth, Gauhar Rehman, Wazir Muhammad, Muawya Elhadi, Afraa Alotaibi, Said Karim Shah
Silver nanoparticles
(Ag-NPs) were synthesized by using
the polyol
method. The structural and morphological characteristics of Ag-NPs
were studied by using X-ray diffraction (XRD) and field-emission scanning
electron microscopy (FE-SEM). The XRD analysis revealed the formation
of single-phase polycrystalline Ag-NPs with an average crystallite
size and lattice constant of ∼23 nm and 4.07 Å, respectively,
while the FE-SEM shows the formation of a uniform and spherical morphology.
Energy-dispersive X-ray spectroscopy confirmed the formation of single-phase
Ag-NPs, and no extra elements were detected. A strong absorption peak
at ∼427 nm was observed in the UV–vis spectrum, which
reflects the surface plasmon resonance (SPR) behavior characteristic
of Ag-NPs with a spherical morphology. Fourier-transform infrared
(FTIR) spectra also supported the XRD and EDX results with regard
to the purity of the prepared Ag-NPs. Anti-inflammatory activity was
tested using HRBCs membrane stabilization and heat-induced hemolysis
assays. The antibacterial activity of Ag-NPs was evaluated against
four different types of pathogenic bacteria by using the disc diffusion
method (DDM). The Gram-negative bacterial strains used in this study
are Escherichia coli (E. coli), Klebsiella, Shigella, and Salmonella. The
analysis suggested that the antibacterial activities of Ag-NPs have
an influential role in inhibiting the growth of the tested Gram-negative
bacteria, and thus Ag-NPs can find a potential application in the
pharmaceutical industry.