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Download fileIntramolecular Coreaction Accelerated Electrochemiluminescence of Polypeptide-Biomineralized Gold Nanoclusters for Targeted Detection of Biomarkers
journal contribution
posted on 2020-06-23, 11:35 authored by Yue Jia, Shanghua Liu, Yu Du, Lei Yang, Xuejing Liu, Lei Liu, Xiang Ren, Qin Wei, Huangxian JuThe development of label-free electrochemiluminescence
(ECL)-based
sensing technology for biomarkers detection has a congenital defect
compared to noncompetitive sandwich-type biosensors due to the lack
of detection antibody conjugated with a signal label. Nevertheless,
it is still not difficult to realize the ultrasensitive analysis benefit
from the exploration of efficient sensing substrates and signal transducers.
In this work, an innovative sensing system is purposed utilizing Fe2O3 nanoarrays (Fe2O3 NAs)
as a well-ordered coreaction accelerator and polypeptide-biomineralized
gold nanoclusters (Au NCs) as a signal transducer. Bifunctional peptide
ligands of H2N-MMYYHFRRHL-COOH (MYH-10) are self-designed;
it cannot only play a role of reductant and coupling reagent for cluster
formation using the MMYY sequence root in the N-terminal but also
act as a connection for coupling carriers and immune molecules via
the HFRRHL region of the C-terminal. In addition to intramolecular
ECL emission between Au NCs and tris(3-aminoethyl)amine (TAEA), all
strategies undoubtedly reduce the spatial hindrance of the sensing
interface and increase the effectiveness of the electron transfer
and immune recognition. With CYFRA21-1 as a target, the biosensor
exhibits a linear ECL response in a wide range (10 fg mL–1 to ∼100 ng mL–1) and an ultralow detection
limit of 1.33 fg mL–1 (S/N = 3). With convincing
experimental data, these innovative strategies will be more eye-catching
in peptide-based nanocluster synthesis and expansion of a more novel
thought for sensing platform fabrication.
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Keywords
detection antibodyultrasensitive analysis benefitIntramolecular CoreactionMYHlabel-free electrochemiluminescenceplatform fabricationintramolecular ECL emissionCYFRA 21-1ultralow detection limitFe 2 O 3 NAsbiomarkers detectionpolypeptide-biomineralized gold nanoclusterselectron transferPolypeptide-Biomineralized Gold NanoclustersNCH 2 N-MMYYHFRRHL-COOHstrategyBifunctional peptide ligandsbiosensor exhibitswell-ordered coreaction acceleratorTAEApeptide-based nanocluster synthesisECL responseFe 2 O 3 nanoarrayssignal transducersHFRRHL regionsignal transducerTargeted DetectionmLMMYY sequence rootsignal labelfgcluster formation