Intracellular Adaptation of Brucella abortus

Lamontagne, Julie; Forest, Anik; Marazzo, Elena; Denis, François; Butler, Heather; Michaud, Jean-François; Boucher, Lyne; Pedro, Ida; Villeneuve, Annie; Sitnikov, Dmitri; Trudel, Karine; Nassif, Najib; Boudjelti, Djamila; Tomaki, Fadi; Chaves-Olarte, Esteban; Guzmán-Verri, Caterina; Brunet, Sylvain; Côté-Martin, Alexandra; Hunter, Joanna; Moreno, Edgardo; Paramithiotis, Eustache

doi:10.1021/pr800978p.s001

Intracellular Adaptation of Brucella abortus

journal contribution

posted on 2009-03-06, 00:00 authored by Julie Lamontagne, Anik Forest, Elena Marazzo, François Denis, Heather Butler, Jean-François Michaud, Lyne Boucher, Ida Pedro, Annie Villeneuve, Dmitri Sitnikov, Karine Trudel, Najib Nassif, Djamila Boudjelti, Fadi Tomaki, Esteban Chaves-Olarte, Caterina Guzmán-Verri, Sylvain Brunet, Alexandra Côté-Martin, Joanna Hunter, Edgardo Moreno, Eustache Paramithiotis

Macrophages were infected with virulent Brucella abortus strain 2308 or attenuated strain 19. Intracellular bacteria were recovered at different times after infection and their proteomes compared. The virulent strain initially reduced most biosynthesis and altered its respiration; adaptations reversed later in infection. The attenuated strain was unable to match the magnitude of the virulent strain’s adjustments. The results provide insight into mechanisms utilized by Brucella to establish intracellular infections.