ao0c02893_si_001.pdf (390.71 kB)
Internalization of Garlic-Derived Nanovesicles on Liver Cells is Triggered by Interaction With CD98
journal contribution
posted on 2020-09-02, 18:04 authored by Heliang Song, Brandon S. B. Canup, Vu L. Ngo, Timothy L. Denning, Pallavi Garg, Hamed LarouiThe
mechanism of how plant-derived nanovesicles are uptaken by
cells remains unknown. In this study, the garlic-derived nanovesicles
(GDVs) were isolated and digested with trypsin to remove all surface
proteins. Digested GDVs showed less uptake compared to undigested
GDVs, confirming that the surface proteins played a role in the endocytosis.
On the cell side (HepG2), interestingly, blocking the CD98 receptors
significantly reduced the uptake of GDVs. During the cellular internalization
of GDVs, we observed that some surface proteins of GDVs were co-localized
with CD98. A total lysate of the GDV surface showed a high presence
of a mannose-specific binding protein, II lectin. Blocking GDV II
lectin (using mannose preincubation) highly reduced the GDV internalization,
which supports that direct interaction between II lectin and CD98
plays an important role in internalization. The GDVs also exhibited in vitro anti-inflammatory effect by downregulating proinflammatory
factors on the HepG2 cells. This work contributes to understanding
a part of the GDV internalization process and the cellular anti-inflammatory
effects of garlic.