Interactions between Anticancer trans-Platinum Compounds and Proteins: Crystal Structures and ESI-MS Spectra of Two Protein Adducts of trans-(Dimethylamino)(methylamino)dichloridoplatinum(II)
journal contributionposted on 04.08.2014, 00:00 by Luigi Messori, Tiziano Marzo, Elena Michelucci, Irene Russo Krauss, Carmen Navarro-Ranninger, Adoracion G. Quiroga, Antonello Merlino
The adducts formed between trans-(dimethylamino)(methylamino)dichloridoplatinum(II), [t-PtCl2(dma)(ma)], and two model proteins, i.e., hen egg white lysozyme and bovine pancreatic ribonuclease, were independently characterized by X-ray crystallography and electrospray ionization mass spectrometry. In these adducts, the PtII center, upon chloride release, coordinates either to histidine or aspartic acid residues while both alkylamino ligands remain bound to the metal. Comparison with the cisplatin derivatives of the same proteins highlights for [t-PtCl2(dma)(ma)] a kind of biomolecular metalation remarkably different from that of cisplatin.
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adductcoordinatecisplatin derivativesbiomolecular metalationchloride releasecrystallographykindCrystal Structurespancreatic ribonucleaseCompoundInteractionSpectraProteinsPtII centeraspartic acid residueshistidinei.eAnticancerProtein Adductsalkylamino ligandslysozymetranelectrospray ionization mass spectrometrymodel proteins