Integration of Magnetic-Field-Directed Self-Assembly-Based
Cell Culture and Biosensing Platform for Improving hPSCs-Derived Neurons
and Quantitative Detection of Neurotransmitter
posted on 2023-12-08, 13:34authored byYufan Zhang, Fan Cao, Min Xu, Xinrui Li, Mengdan Tao, Shanshan Wu, Wei Xu, Yan Liu, Wanying Zhu
Despite
the fact that human neural cell models have played significant
roles in both research and cell replacement therapies for neurological
diseases, the existing techniques for obtaining neurons from human
pluripotent stem cells (hPSCs) are arduous and intricate. Additionally,
the evaluation of neuron quality in the natural environment remains
deficient. Consequently, we have developed a comprehensive platform
utilizing magnetic-field-directed self-assembly (MDSA) of MXenes@Fe3O4 (M/F) nanocomposites. This platform facilitates
the cultivation and in situ analysis of differentiated dopaminergic
(DA) neurons. Our results showed that the introduction of M/F enhances
neurite outgrowth and leads to the development of more intricate ramifications.
Moreover, with the increase of magnetic field intensity, neurite outgrowth
is further enhanced, and the proportion of differentiated mature neurons
from hPSCs increases. This suggests that our platform promotes the
maturation of neurons, emphasizing the crucial role of biophysical
cues in expediting the differentiation process. The homogenization
platform formed by MDSA of M/F nanocomposites exhibits high conductivity,
leading to its exceptional performance in the real-time monitoring
of the release of dopamine neurotransmitter from hPSC-derived DA neurons.
Hence, this platform demonstrates significant potential for monitoring
cell quality. In conclusion, our integrated platform, based on MDSA
of M/F nanocomposites, offers a reliable and efficient means for the
in vitro generation of human neurons with a controllable quality.
The as-prepared platform holds potential for enhancing neuronal maturation
and ensuring consistent cell quality, showing significant implications
for in vitro biological research, disease modeling, and cell replacement
therapy.