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Download fileInstrument-Free Synthesizable Fabrication of Label-Free Optical Biosensing Paper Strips for the Early Detection of Infectious Keratoconjunctivitides
journal contribution
posted on 2016-04-29, 00:00 authored by Wansun Kim, Jae-Chul Lee, Jae-Ho Shin, Kyung-Hyun Jin, Hun-Kuk Park, Samjin ChoiWe
introduce a surface-enhanced Raman scattering (SERS)-functionalized,
gold nanoparticle (GNP)-deposited paper strip capable of label-free
biofluid sensing for the early detection of infectious eye diseases.
The GNP biosensing paper strip was fabricated by the direct synthesis
and deposition of GNPs on wax-divided hydrophilic areas of a permeable
porous substrate through a facile, power-free synthesizable, and highly
reproducible successive ionic layer absorption and reaction (SILAR)
technique. To maximize localized surface plasmon resonance-generated
SERS activity, the concentration of the reactive solution and number
of SILAR cycles were optimized by controlling the size and gap distance
of GNPs and verified by computational modeling with geometrical hypotheses
of Gaussian-estimated metallic nanoparticles. The responses of our
SERS-functionalized GNP paper strip to Raman intensities exhibited
an enhancement factor of 7.8 × 108, high reproducibility
(relative standard deviation of 7.5%), and 1 pM 2-naphthalenethiol
highly sensitive detection limit with a correlation coefficient of
0.99, achieved by optimized SILAR conditions including a 10/10 mM/mM
HAuCl4/NaBH4 concentration and six SILAR cycles.
The SERS-functionalized GNP paper is supported by a multivariate statistics-preprocessed
machine learning-judged bioclassification system to provide excellent
label-free chemical structure sensitivity for identifying infectious
keratoconjunctivitis. The power-free synthesizable fabrication, label-free,
rapid analysis, and high sensitivity feature of the SILAR-fabricated
SERS-functionalized GNP biosensing paper strip makes it an excellent
alternative in point-of-care applications for the early detection
of various infectious diseases.