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Insights into the Soluble PEG-Supported Synthesis of Cytosine-Containing Nucleoside 5′-Mono-, Di-, and Triphosphates

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journal contribution
posted on 04.12.2009, 00:00 authored by Céline Crauste, Christian Périgaud, Suzanne Peyrottes
Nucleoside phosphoesters (nucleotides) have crucial roles in a large variety of biological processes including nucleic acid biosynthesis and their corresponding analogues are extensively used as biological tools. Herein, we describe a new and efficient synthetic procedure involving polyethylene glycol (PEG) as soluble support and regioselective mono-, di-, and triphosphorylation steps. Applied to natural and synthetic cytosine containing nucleosides, this methodology allowed the preparation of various phosphorylated forms in high yields and good purity.

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