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Insights in the Organization of DNA−Surfactant Monolayers Using Cryo-Electron Tomography

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journal contribution
posted on 03.10.2007, 00:00 by Matthijn R. J. Vos, Paul H. H. Bomans, Felix de Haas, Peter M. Frederik, John A. Jansen, Roeland J. M. Nolte, Nico A. J. M. Sommerdijk
We demonstrate that for the present system the general view on DNA−cationic surfactant Langmuir monolayers, in which DNA is bound directly underneath a closed monolayer surface, is only applicable when DNA is injected underneath a preformed monolayer. Moreover, cryo-electron tomography showed that the DNA strands do not bind completely to the monolayer surface in an orderly fashion, but partially extend down into the subphase.