am5b00481_si_001.pdf (2.04 MB)
Injection Molded Polymeric Micropatterns for Bone Regeneration Study
journal contribution
posted on 2015-04-08, 00:00 authored by Erika Zanchetta, Enrica Guidi, Gioia Della Giustina, Marco Sorgato, Mauro Krampera, Giulio Bassi, Rosa Di Liddo, Giovanni Lucchetta, Maria Teresa Conconi, Giovanna BrusatinAn industrially feasible process
for the fast mass-production of molded polymeric micro-patterned substrates
is here presented. Microstructured polystyrene (PS) surfaces were
obtained through micro injection molding (μIM) technique on
directly patterned stamps realized with a new zirconia-based hybrid
spin-on system able to withstand 300 cycles at 90 °C. The use
of directly patterned stamps entails a great advantage on the overall
manufacturing process as it allows a fast, flexible, and simple one-step
process with respect to the use of milling, laser machining, electroforming
techniques, or conventional lithographic processes for stamp fabrication.
Among the different obtainable geometries, we focused our attention
on PS replicas reporting 2, 3, and 4 μm diameter pillars with
8, 9, 10 μm center-to-center distance, respectively. This enabled
us to study the effect of the substrate topography on human mesenchymal
stem cells behavior without any osteogenic growth factors. Our data
show that microtopography affected cell behavior. In particular, calcium
deposition and osteocalcin expression enhanced as diameter and interpillar
distance size increases, and the 4-10 surface was the most effective
to induce osteogenic differentiation.
History
Usage metrics
Categories
Keywords
substrate topographycalcium depositionmanufacturing processcell behavior4 μ m diameter pillarselectroforming techniqueslaser machiningosteocalcin expressioninterpillar distance size increasesMicrostructured polystyreneinjection Molded Polymeric Micropatternsosteogenic differentiation300 cyclescells behaviorosteogenic growth factorssurfacestamp fabricationBone Regeneration StudyAnIMdata showPS replicas