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Inhibitory Effect of Condensed Tannins from Banana Pulp on Cholesterol Esterase and Mechanisms of Interaction
journal contribution
posted on 2019-12-10, 20:35 authored by Xiangxin Li, Haitao Jiang, Yijing Pu, Jiankang Cao, Weibo JiangIn the present study, the inhibitory effect of condensed
tannins
(CTs) on cholesterol esterase (CEase) was studied. The underlying
mechanisms were evaluated by reaction kinetics, turbidity and particle
size analyses, multispectroscopy methods, thermodynamics, and computer
molecular simulations. CTs showed potent CEase inhibitory activity
with an IC50 value of 64.19 μg/mL, and the CEase
activity decreased with increasing CT content in a mixed-competitive
manner, which was verified by molecular docking simulations. Fluorescence
and UV–vis measurements revealed that complexes were formed
from CEase and CTs by noncovalent interaction. Isothermal titration
calorimetry indicated that the interaction between CEase and CTs occurred
through hydrogen bonding and hydrophobic interactions. Circular dichroism
analysis suggested that CTs inhibited the activity of CEase by altering
the secondary structure of CEase. The inhibition of CTs on CEase in
the gastrointestinal tract might be one mechanism for its cholesterol-lowering
effect.
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Keywords
noncovalent interactionInhibitory Effectparticle size analysesCondensed TanninsCEase activitydocking simulationsIsothermal titration calorimetrycholesterol esterasereaction kineticsCircular dichroism analysisCT contentmixed-competitive mannerIC 50 valueUVCholesterol EsteraseBanana Pulpmultispectroscopy methods
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