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Download fileIn Vivo Selective Capture and Rapid Identification of Luteolin and Its Metabolites in Rat Livers by Molecularly Imprinted Solid-Phase Microextraction
journal contribution
posted on 2017-01-23, 00:00 authored by Die Gao, Dan-Dan Wang, Qian Zhang, Feng-Qing Yang, Zhi-Ning Xia, Qi-Hui Zhang, Chun-Su YuanA method based on molecularly imprinted
solid-phase microextraction
(MIP-SPME) coupled with liquid chromatography-quadrupole time-of-flight
tandem mass spectrometry (QTOF-MS/MS) was developed for the detection
of luteolin and its metabolites in vivo. The MIP-SPME fibers were
first fabricated by dopamine and silane, and then luteolin MIPs-coated
fibers were successfully prepared using luteolin, acrylamide (AM),
and ethylene glycol dimethacrylate (EGDMA) as the template, functional
monomer and cross-linker, respectively. The characterizations of polymers
were analyzed by scanning electron microscopy (SEM), Fourier transform
infrared spectroscopy (FT-IR), and the Brunauer–Emmett–Teller
method (BET). The properties involving adsorption and selective experiments
were evaluated, and these results revealed that MIP fibers presented
high adsorption capacity and selectivity to luteolin. Furthermore,
the developed MIP-SPME coupled with the LC-QTOF-MS/MS method was adopted
to capture and identify luteolin and its metabolites in rat livers
in vivo, and eventually, apigenin, chrysoeriol, and diosmetin were
rapidly identified as metabolites.
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Keywords
MIP-SPME fibersBETethylene glycol dimethacrylateadsorption capacityRat LiversLC-QTOF-MSrat liversSEMmethodQTOF-MSchromatography-quadrupole time-of-flight tandem mass spectrometryFT-IRAMEGDMARapid IdentificationMolecularly Imprinted Solid-Phase Microextractionmetabolitesolid-phase microextractionvivoMIP fibersscanning electron microscopyluteolin MIPs-coated fibers