American Chemical Society
ac7b03270_si_001.pdf (1.48 MB)

In Vivo Oxidative Stress Monitoring Through Intracellular Hydroxyl Radicals Detection by Recyclable Upconversion Nanoprobes

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journal contribution
posted on 2017-11-07, 00:00 authored by Yuxin Liu, Qi Jia, Quanwei Guo, Anqi Jiang, Jing Zhou
Oxidative stress, as an essential cause to many diseases via irreversible biomolecule oxidation, can be induced by casual-contacted nanomaterials, which can generate hydroxyl radical (OH) due to their active surface capacity. Herein, we report a novel upconversion nanoprobe for ratiometric OH detection and monitoring titanium oxide nanomaterial-induced oxidative stress. Unlike previously developed nanoprobes, the OH-responsive acceptor in these nanoprobes can be rapidly prepared and easily recycled. With a detection limit of ∼2 nM and a broad linear range of 4 nM–16 μM, the nanoprobe is suitable for both in vitro and in vivo applications. The facile preparation and recyclable strategy provide a new method for developing novel nanoprobes that can directly detect hazardous molecules in biological samples and systems.