ja7b07241_si_001.pdf (1.78 MB)
In Vitro Selection of Diversely Functionalized Aptamers
journal contribution
posted on 2017-09-22, 00:00 authored by Dehui Kong, Wayland Yeung, Ryan HiliWe describe the application of T4
DNA ligase-catalyzed DNA templated
oligonucleotide polymerization toward the evolution of a diversely
functionalized nucleic acid aptamer for human α-thrombin. Using
a 256-membered ANNNN comonomer library comprising 16 sublibraries
modified with different functional groups, a highly functionalized
aptamer for thrombin was raised with a dissociation constant of 1.6
nM. The aptamer was found to be selective for thrombin and required
the modifications for binding affinity. This study demonstrates the
most differentially functionalized nucleic acid aptamer discovered
by in vitro selection and should enable the future exploration of
functional group dependence during the evolution of nucleic acid polymer
activity.