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Improved Synthesis of 2‑Trifluoromethyl-10-aminopropylphenothiazine: Making 2‑Trifluoromethyl-10-aminopropylphenothiazine Readily Available for Calmodulin Purification

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journal contribution
posted on 30.11.2018, 12:18 by Josiah W. Johnson, Kyle W. Cain, Tori B. Dunlap, Gregory R. Naumiec
An improved and high yielding three-step synthesis for the production of 2-trifluoromethyl-10-aminopropylphenothiazine (TAPP) using less hazardous and more inexpensive reagents, its coupling to Sepharose-4B resin, and its ability to purify calmodulin are described. The overall yield of TAPP, starting with 3-aminopropyl bromide hydrobromide and 2-(trifluoromethyl)­phenothiazine, was 96%.