Immunosensing of Atrazine with Antibody-Functionalized Cu-MOF Conducting Thin Films
journal contributionposted on 02.12.2015, 00:00 by Sanjeev K. Bhardwaj, Neha Bhardwaj, Girish C. Mohanta, Pawan Kumar, Amit L. Sharma, Ki-Hyun Kim, Akash Deep
This work reports the assembly of thin films of a silica (SiO2)-modified copper–metal organic framework, Cu3(BTC)2 [Cu3(BTC)2@SiO2, BTC = benzene-1,3,5-tricarboxylic acid] on a conducting substrate of NH2–BDC [NH2–BDC = 2-aminobenzene-1,4-dicarboxylic acid] doped polyaniline (PANI). Assembled Cu3(BTC)2@SiO2/BDC-PANI thin films displayed electrical conductivity in the range of 35 μA. These thin films were conjugated with antiatrazine antibodies to create a novel immunosensing platform. Various structural and spectral characteristics of the synthesized material and its bioconjugate were investigated. The developed immunosensor was used for the conductometric sensing of atrazine. The detection of atrazine was achieved with a high sensor sensitivity (limit of detection = 0.01 nM) and specificity in the presence of diverse pesticides (e.g., endosulfan, parathion, paraoxon, malathion, and monochrotophos).