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Immunoglobulin G‑Encapsulated Gold Nanoclusters as Fluorescent Tags for Dot-Blot Immunoassays
journal contribution
posted on 2019-08-23, 22:43 authored by Quan-Quan Zhuang, Hao-Hua Deng, Shao-Bin He, Hua-Ping Peng, Zhen Lin, Xing-Hua Xia, Wei ChenFew-atom
gold nanoclusters (AuNCs) have been fabricated and used
for various fields owing to their remarkable optical and photophysical
features. However, the rational design for the antibody-mediated synthesis
of fluorescent AuNCs for direct antigen–antibody reactions
remains unexplored. In this work, immunoglobulin G (IgG)-functionalized
AuNCs (IgG-AuNCs) were successfully prepared via a facile and fast
biomineralization process. The generated IgG-AuNCs can emit intense
red fluorescence with a high photoluminescence quantum yield. Besides
strong emission, the bioactivity of IgG on the IgG-AuNCs can be retained.
Surface plasmon resonance measurements suggested that IgG-AuNCs can
bind to goat anti-human IgG with an affinity constant of 6.21 ×
10–8 M. A simple detection method was then developed
using a dot-blot immunoassay with IgG-AuNCs as fluorescent tags. Experimental
results confirmed that the IgG-AuNC-based fluorescent reporters had
many advantages such as low nonspecific adsorption and good photostability,
offering immense potential for the development of efficient biosensors.
This work can be extended to other specific antibodies to produce
multifunctional AuNCs and utilized to detect and monitor targeted
analytes and biological events of interest.
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Keywords
photophysical featuresbiomineralization processmultifunctional AuNCsSurface plasmon resonance measurementsantibody-mediated synthesisIgGdetection methodDot-Blot Immunoassays Few-atom gold nanoclustersimmunoglobulin Gphotoluminescence quantumFluorescent Tagsdot-blot immunoassayExperimental resultsIgG-AuNC
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