Immobilizing Redox Enzyme on Amino Functional Group-Integrated
Tailor-Made Polyester Textile: High Loading, Stability, and Application
in a Bio-Fenton System
posted on 2021-06-25, 10:03authored byMohammad Neaz Morshed, Nemeshwaree Behary, Jinping Guan, Vincent A. Nierstrasz
This study reports
the first approach of immobilizing a redox (glucose
oxidase-GOx) enzyme on the amino functional group-integrated tailor-made
textile (polyester nonwoven fabric-PF) support matrix. To achieve
that, polyethylenimine if not chitosan was chemically grafted on plasma
(with O2/N2 gas)-activated PF before immobilizing
the GOx enzyme through physical adsorption. Diverse qualitative and
quantitative characterization methods were used to validate the successful
activation and GOx immobilization on amino functional group-integrated
tailor-made PF. Results showed that integration of amino functional
groups on PF offers a great deal of favorable conditions during enzyme
immobilization through covalent or ionic interaction between counter
functional groups as reflected in high loading (55.46%) and good operational
(78.37%) and thermal stability (∼60 °C) with excellent
recyclability (60% activity/15-cycles) and poor leaching (22%) of
immobilized GOx. Enzymatic reaction kinetics of free and immobilized
GOx revealed the existence of relative mass transfer and diffusion
limitation of immobilized GOx as apprehended in the apparent Michaelis
constant (Km) and maximum velocity of
the reaction (Vmax). The resultant immobilized
GOx’s were studied for the first time in the removal of pollutants
(10 mg L–1 crystal violet) from water in a heterogeneous
bio-Fenton system. Results showed as high as 88.69% pollutant removal
at 1.19 × 10–2 min–1 following
pseudo-first-order kinetic model as supported by R2 values beyond 97. These results are of great importance
as they provide fundamental evidence and proof of concepts regarding
immobilizing biocatalysts on textiles and their potential application
in a robust heterogeneous catalytic system for environmental and green
chemistry applications.