posted on 2024-02-02, 11:19authored byAlvaro Villanueva, Fernando Rivero-Pino, Maria E. Martin, Teresa Gonzalez-de la Rosa, Sergio Montserrat-de la Paz, Maria C. Millan-Linares
The incorporation
of novel, functional, and sustainable foods in
human diets is increasing because of their beneficial effects and
environmental-friendly nature. Chia (Salvia hispanica L.) has proved to be a suitable source of bioactive peptides via
enzymatic hydrolysis. These peptides could be responsible for modulating
several physiological processes if able to reach the target organ.
The bioavailable peptides contained in a hydrolysate obtained with
Alcalase, as functional foods, were identified using a transwell system
with Caco-2 cell culture as the absorption model. Furthermore, 20
unique peptides with a molecular weight lower than 1000 Da and the
higher statistical significance of the peptide-precursor spectrum
match (−10 log P) were assessed by in silico
tools to suggest which peptides could be those exerting the demonstrated
bioactivity. From the characterized peptides, considering the molecular
features and the results obtained, the peptides AGDAHWTY, VDAHPIKAM,
PNYHPNPR, and ALPPGAVHW are anticipated to be contributing to the
antioxidant and/or ACE inhibitor activity of the chia protein hydrolysates.