American Chemical Society
ja072027p_si_001.pdf (320.8 kB)

Identification of a Peptoid Inhibitor of the Proteasome 19S Regulatory Particle

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journal contribution
posted on 2007-06-27, 00:00 authored by Hyun-Suk Lim, Chase T. Archer, Thomas Kodadek
The first chemical inhibitor of the 19S regulatory particle (RP) of the proteasome is described. The molecule was identified by screening a library of nucleoside-capped peptoids for binding to the yeast 26S proteasome in a crude extract. The hit was resynthesized and shown to block 19S RP-mediated protein unfolding in vitro and proteasome-mediated turnover of p27 in HeLa cells.