Identification of Antiadipogenic Constituents of the Rhizomes of Anemarrhena asphodeloides

Three new phenolic compounds, (E)-4′-demethyl-6-methyleucomin (1), anemarcoumarin A (2), and anemarchalconyn (3), were isolated from an ethyl acetate extract of the rhizomes of Anemarrhena asphodeloides, together with seven known compounds (4−10). The structures of the new compounds (1−3) were determined on the basis of spectroscopic data interpretation. Compound 3 exhibited a potent inhibitory effect against the differentiation of preadipocyte 3T3-L1 cells with an IC₅₀ value of 5.3 μM.