posted on 2023-11-10, 14:03authored byArnab Dey, Benoît Charrier, Victor Ribay, Jean-Nicolas Dumez, Patrick Giraudeau
The
application of NMR spectroscopy to complex mixture analysis
and, in particular, to metabolomics is limited by the low sensitivity
of NMR. We recently showed that dissolution dynamic nuclear polarization
(d-DNP) could enhance the sensitivity of 13C NMR for complex
metabolite mixtures, leading to the detection of highly sensitive 13C NMR fingerprints of complex samples such as plant extracts
or urine. While such experiments provide heteronuclear spectra, which
are complementary to conventional NMR, hyperpolarized 1H NMR spectra would also be highly useful, with improved limits of
detection and compatibility with the existing metabolomics workflow
and databases. In this technical note, we introduce an approach capable
of recording both 1H and 13C hyperpolarized
spectra of metabolite mixtures in a single experiment and on the same
hyperpolarized sample. We investigate the analytical performance of
this method in terms of sensitivity and repeatability, and then we
show that it can be efficiently applied to a plant extract. Significant
sensitivity enhancements in 1H NMR are reported with a
repeatability suitable for metabolomics studies without compromising
on the performance of hyperpolarized 13C NMR. This approach
provides a way to perform both 1H and 13C hyperpolarized
NMR metabolomics with unprecedented sensitivity and throughput.