posted on 2020-03-23, 19:03authored byAndré
N. C. de Barros, Emanoela F. Q. Santos, Dasciana S. Rodrigues, Raquel L. C. Giordano, Thiago F. de Pádua
The hydrophobic adsorption is an
alternative to traditional organic
solvent extraction for the recovery and purification of Penicillin
G (PenG). However, there is a lack of information concerning the effect
of process variables and technical feasibility while balancing product
degradation. After assessing the integrity of PenG under different
conditions, Amberlite XAD-4 was selected from among three different
adsorbents. During the batch process using only 0.05 gXAD‑4/mLmedium, the adsorption yield increased from 36% at
pH 6 to 44% at pH 4. More than 90% of the antibiotic was captured
from the fermentation broth using 0.083 gXAD‑4/mLmedium in a 45 min batch performed at pH 4 and 4 °C. Moreover,
there was no PenG degradation. The desorption conditions were evaluated,
and 95% of the antibiotic could be recovered in only one batch using
water–ethanol, which is an unexplored PenG desorption process.
The results showed selective adsorption, indicating that the process
can also be useful for purification purposes. Hydrophobic adsorption
with ethanol desorption is efficient, scalable, and green and could
be used in place of traditional methods or in extractive fermentation.