Human Gastrointestinal Metabolism of the Cistanches
Herba Water Extract in Vitro: Elucidation of the Metabolic Profile
Based on Comprehensive Metabolite Identification in Gastric Juice,
Intestinal Juice, Human Intestinal Bacteria, and Intestinal Microsomes
posted on 2017-08-03, 00:00authored byYang Li, Ying Peng, Mengyue Wang, Pengfei Tu, Xiaobo Li
Cistanches Herba is taken orally
as a health food supplement and
medicinal plant in Asian countries. It consists of the stems of Cistanche deserticola (CD) and Cistanche tubulosa (CT). The gastrointestinal metabolism of the multiple components
contained in Cistanches Herba is crucial for the discovery of bioactive
constituents. This study aims to elucidate the comprehensive metabolic
profile of the Cistanches Herba water extract by simulating human
gastrointestinal metabolism in vitro independently and sequentially
using four models: gastric juice, intestinal juice, human intestinal
bacteria, and human intestinal microsomes. A total of 35 and 18 metabolites
were characterized from CD and CT water extracts, respectively. These
metabolites were formed through reduction, methylation, dimethylation,
deglycosylation, decaffeoyl,
derhamnose, dehydrogenation, and glucuronidation. The
difference in metabolites of the Cistanches Herba water extract and
single compounds and the difference in metabolites of CD and CT water
extracts were caused by the oligosaccharides and polysaccharides in
Cistanches Herba.