posted on 2021-10-14, 04:04authored byQingqing Zhang, Suying Liu, Cuicui Du, Yamin Fu, Ke Xiao, Xiaohua Zhang, Jinhua Chen
Highly sensitive and selective microRNA
(miRNA) assay is of great
significance for disease diagnosis and therapy. Herein, a magnetic-assisted
electrochemistry (EC)–photoelectrochemistry (PEC) dual-mode
biosensing platform was developed for miRNA-210 detection based on
dual-signaling EC and photocurrent-polarity-switching PEC strategies.
Porous magnetic Fe3O4 octahedra with a large
surface area were synthesized by calcining Fe-based metal–organic
frameworks. Subsequently, the magnetic photoelectric materials (Fe3O4@CdS) were developed by the successive ionic
layer adsorption and reaction method in Cd2+ and S2– solutions. Then, the self-assembled DNA nanoprisms
contained three thiols/hanging arms that could capture miRNA-210 efficiently
and were anchored to the Fe3O4@CdS octahedra
via the Cd–S bond. When miRNA-210 was present, the double-stranded
DNA concatemers [the self-assembled duplex helixes based on a pair
of methylene blue (MB)-labeled single-stranded DNAs (AP1 and AP2)
through the hybridization chain reaction and then intercalated with
adriamycin (Dox) into their grooves] were connected with the Fe3O4@CdS-DNA nanoprisms. MB and Dox not only acted
as the electrochemical probes but also synergistically switched the
photocurrent polarity of the Fe3O4@CdS octahedra.
Thus, miRNA-210 was assayed sensitively and selectively via the proposed
EC–PEC dual-mode biosensing platform. Additionally, the abovementioned
recognition steps occurred in a homogeneous system, and the effects
of the impurities and interferences on the miRNA-210 assay could be
easily avoided by magnetic separation due to the good magnetic properties
of Fe3O4 octahedra. The proposed EC–PEC
dual-mode biosensing platform showed a wide range of potential applications
in bioanalysis and early diagnosis of disease.