jm7b01402_si_001.pdf (796.51 kB)
Highly Selective, Potent, and Oral mTOR Inhibitor for Treatment of Cancer as Autophagy Inducer
journal contributionposted on 2018-01-08, 00:00 authored by Qingxiang Guo, Chenhua Yu, Chao Zhang, Yongtao Li, Tianqi Wang, Zhi Huang, Xin Wang, Wei Zhou, Yao Li, Zhongxiang Qin, Cheng Wang, Ruifang Gao, Yongwei Nie, Yakun Ma, Yi Shi, Jianyu Zheng, Shengyong Yang, Yan Fan, Rong Xiang
On the basis of novel pyrazino[2,3-c]quinolin-2(1H)-one scaffold, we designed and identified a highly selective, potent and oral mTOR inhibitor, 9m. Compound 9m showed low nanomolar activity against mTOR (IC50 = 7 nM) and greater selectivity over the related PIKK family kinases, which demonstrated only modest activity against 3 out of the 409 protein kinases. In vitro assays, compound 9m exhibited high potency against human breast and cervical cancer cells and induced tumor cell cycle arrest and autophagy. 9m inhibited cellular phosphorylation of mTORC1 (pS6 and p4E-BP1) and mTORC2 (pAKT (S473)) substrates. In T-47D xenograft mouse model, oral administration of compound 9m led to significant tumor regression without obvious toxicity. In addition, this compound showed good pharmacokinetics. Collectively, due to its high potency and selectivity, compound 9m could be used as a mTOR drug candidate.