Highly Selective Detection of Iodide in Biological, Food, and Environmental Samples Using Polymer-Capped Silver Nanoparticles: Preparation of a Paper-Based Testing Kit for On-Site Monitoring

This work describes a facile synthesis of polymer-capped silver nanoparticles at room temperature. Chitosan oligosaccharide lactate-capped silver nanoparticles (COL-AgNPs) show the surface plasma resonance (SPR) band at 400 nm. The color of the COL-AgNPs was observed to be brownish yellow. The synthesized COL-AgNPs are stable for 5 months. The COL-AgNPs were characterized by UV–vis, X-ray diffraction, high-resolution transmission electron microscopy (HR-TEM), mass, and Fourier transform infrared spectral techniques. The obtained COL-AgNPs are monodispersed, and the range of the particle diameter was calculated to be 16.37 ± 0.15 nm by HR-TEM. We have utilized the COL-AgNPs as a probe to sense iodide (I^–). The SPR band of COL-AgNPs was decreased after the addition of iodide, and the color of the solution changed to colorless. Based on the decreases in SPR band absorbance, the concentration of iodide was calculated. The detection limit was found to be 108.5 × 10^–9 M (S/N = 3). Other interferences (825- and 405-fold) did not interfere with the detection of 1.48 × 10^–6 M iodide. The sensing mechanism was also discussed. Finally, we have successfully applied our sensing system for the detection of iodide in tap water, river water, pond water, blood serum, urine, and food samples. Good recoveries are obtained with spiked iodide in the real samples. Importantly, we have developed a paper-based kit using wax-printed paper for the on-site monitoring of iodide. The developed paper-based kit absorbance was validated with the microplate reader. To the best of our knowledge, this is the first report that used six different real samples for the detection of iodide and development of the paper-based kit for on-site monitoring.