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Highly Efficient Enrichment of O‑GalNAc Glycopeptides by Using Immobilized Metal Ion Affinity Chromatography

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journal contribution
posted on 19.05.2021, 16:36 by Xuyang Yue, Hongqiang Qin, Yao Chen, Zheng Fang, Luyao Liu, He Zhu, Xiaoyan Liu, Jiahua Zhou, Kailu Tian, Xiaoqiang Qiao, Mingliang Ye
Proteomics analysis of O-GalNAc glycosylation is important for the screening of biomarkers and the assessment of therapeutic responses. However, its analysis still faces challenges due to the poor performance of currently available enrichment methods. In this study, an enrichment method was established on the basis of Ti-IMAC­(IV) materials, which could enrich the intact O-GalNAc glycopeptides via both the hydrophilic interaction and affinity interaction. This method enabled nearly 200 intact O-GalNAc glycopeptides identified from only 0.1 μL of human serum. This was nearly 2-fold different from that of the HILIC method. An in-depth analysis of the O-GalNAc glycosylation was performed, and 2093 intact glycopeptides were identified from 7.2 μL of human serum samples. This is the largest O-GalNAc glycosylation database of human serum from a trace amount of sample. Furthermore, 52 significantly changed intact O-GalNAc glycopeptides were determined by the quantitative analysis of hepatocellular carcinoma (HCC) and control serum samples, indicating the potential applications of this enrichment method in biomarker discovery.