posted on 2021-04-13, 17:04authored byKendall Hoff, Xun Ding, Lucas Carter, John Duque, Ju-Yu Lin, Samantha Dung, Priyanka Singh, Jiayi Sun, Filip Crnogorac, Radha Swaminathan, Emily N. Alden, Xuechen Zhu, Ryota Shimada, Marijan Posavi, Noah Hull, Darrell Dinwiddie, Adam M. Halasz, Glenn McGall, Wei Zhou, Jeremy S. Edwards
SARS-CoV-2
has infected over 128 million people worldwide, and
until a vaccine is developed and widely disseminated, vigilant testing
and contact tracing are the most effective ways to slow the spread
of COVID-19. Typical clinical testing only confirms the presence or
absence of the virus, but rather, a simple and rapid testing procedure
that sequences the entire genome would be impactful and allow for
tracing the spread of the virus and variants, as well as the appearance
of new variants. However, traditional short read sequencing methods
are time consuming and expensive. Herein, we describe a tiled genome
array that we developed for rapid and inexpensive full viral genome
resequencing, and we have applied our SARS-CoV-2-specific genome tiling
array to rapidly and accurately resequence the viral genome from eight
clinical samples. We have resequenced eight samples acquired from
patients in Wyoming that tested positive for SARS-CoV-2. We were ultimately
able to sequence over 95% of the genome of each sample with greater
than 99.9% average accuracy.