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High Throughput Detection of Human Neutrophil Peptides from Serum, Saliva, and Tear by Anthrax Lethal Factor-Modified Nanoparticles

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journal contribution
posted on 11.09.2013, 00:00 by Yuanyuan Zhao, Xu Zhang, Xiangdong Xue, Zhipeng Li, Fei Chen, Shengliang Li, Anil Kumar, Guozhang Zou, Xing-Jie Liang
Human α defensins human neutrophil peptide 1–3 (HNP 1–3) are potential prognostic cancer biomarkers. Metalloprotein anthrax lethal factor (ALF) binds to HNP 1–3 in a Zn2+-dependent manner. We conjugated ALF to the surface of magnetic nanoparticles (MNP) to magnetically isolate the HNPs, and used Zn2+ to control the capture and release HNPs.